GET IDEAL IMAGE QUALITY
SAVING CELLS FROM PHOTOTOXICITY or BLEACHING
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Schematic cut-away diagram showing a Yokogawa Electric Corporation spinning disk confocal unit.
This unit includes the motorized spinning pinhole disk, as well as a micro-lens disk and can be mounted to the camera port of a microscope to provide confocal images. The unit pictured can accommodate two cameras observing different wavelength channels, due to the internal dichroic beam splitter and filter wheels.
From Zeiss Campus: http://zeiss campus.magnet.fsu.edu/articles/spinningdisk/introduction.html
Microlens disk focuses illumination light through pinholes of primary disk. Returning light from the sample is separated by a dichroic mirror to scan across the sensor of a CMOS or CCD camera.
From Graf, R, J Rietdorf, and T Zimmermann. 2005. “Live Cell Spinning Disk Microscopy.” Adv Biochem Engin/Biotechnol 95: 57-75.
50µm pinholes optimized for high NA objectives
Multi-beam scanning (1 000) increases scanning speed, but also results in significantly lower photobleaching and phototoxicity | Gentler imaging enabling better imaging of delicate specimens
Filter wheel with six filter positions (in high-end model) | Optimal flexibility for multichannel imaging
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Multi-color imaging Long-term
Multi-dimensional live cell imaging
Electrophysiology (neuronal – cardiac – muscle – optogenetics)
Intra-vital imaging (Brain – In-situ vasculature – Blood flow))
spinning disc confocal set up
NeurImag imaging facility – INSERM U894 – IPNP
102-108 rue de la Sante, 75014 Paris
Contact person: Ms Lydia DANGLOT ✉ firstname.lastname@example.org
Fluorescence imaging, Confocal imaging
Inverted microscope capable of confocal imaging, equipped with glycerol immersion objectives.
CSU-X1 Spinning disc
2 Flash 4.0 Hamamatsu cameras
Lasers: 405nm (200mW) – 488nm (180mW) – 561 (300 mW) – 640 (400 mW) – 730 (100 mW)