Systems / Confocal Microscopy Solution
CSU-X1 spinning Disk Confocal
Faster, Brighter, and More Versatile
GET IDEAL IMAGE QUALITY
SAVING CELLS FROM PHOTOTOXICITY or BLEACHING
Faster
Brighter
More versatile
Powered by MetaMorph software platform
Schematic cut-away diagram showing a Yokogawa Electric Corporation spinning disk confocal unit.
This unit includes the motorized spinning pinhole disk, as well as a micro-lens disk and can be mounted to the camera port of a microscope to provide confocal images. The unit pictured can accommodate two cameras observing different wavelength channels, due to the internal dichroic beam splitter and filter wheels.
From Zeiss Campus: http://zeiss campus.magnet.fsu.edu/articles/spinningdisk/introduction.html
Microlens disk focuses illumination light through pinholes of primary disk. Returning light from the sample is separated by a dichroic mirror to scan across the sensor of a CMOS or CCD camera.
From Graf, R, J Rietdorf, and T Zimmermann. 2005. “Live Cell Spinning Disk Microscopy.” Adv Biochem Engin/Biotechnol 95: 57-75.
Up to 1 000 fps (standard) – Fastest speed spinning disk for 3D confocal imaging (micro-lens enhanced Nipkow disk scanning) | No trade-off needed, better accuracy for fast cell dynamics 50µm pinholes optimized for high NA objectives Multi-beam scanning (1 000) increases scanning speed, but also results in significantly lower photobleaching and phototoxicity | Gentler imaging enabling better imaging of delicate specimens Filter wheel with six filter positions (in high-end model) | Optimal flexibility for multichannel imaging Powered by MetaMorph software | Expand CSU-X1 with multi-modal imaging capabilities & performance
# Membrane biology (Vesicle trafficking) Cell motility Cell Division FRET imaging Ion Imaging Sub-cellular dynamics Multi-color imaging Long-term Multi-dimensional live cell imaging Electrophysiology (neuronal – cardiac – muscle – optogenetics) Intra-vital imaging (Brain – In-situ vasculature – Blood flow))
spinning disc confocal set up
NeurImag imaging facility – INSERM U894 – IPNP
102-108 rue de la Sante, 75014 Paris
Contact person: Ms Lydia DANGLOT ✉ lydia.danglot@inserm.fr
Applications
Fluorescence imaging, Confocal imaging
Specifications
Inverted microscope capable of confocal imaging, equipped with glycerol immersion objectives.
CSU-X1 Spinning disc
Hamamatsu Gemini-2C
2 Flash 4.0 Hamamatsu cameras
Lasers: 405nm (200mW) – 488nm (180mW) – 561 (300 mW) – 640 (400 mW) – 730 (100 mW)
Errol-Laser STARSCAN
